Abstract

Seaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed Halimeda tuna. The H. tuna sample was extracted with the maceration method using methanol and then partitioned using ethyl acetate and water to obtain ethyl acetate and water fractions. The methanol extract, ethyl acetate fraction, and water fraction of H. tuna were tested for their inhibitory activity against α-amilase and α-glucosidase. The methanol extract and the fractions with the highest inhibitory activity were phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the ethyl acetate fraction (IC50 = 0.88 ± 0.20 mg/mL) inhibited α-amylase relatively similar to acarbose (IC50 = 0.76 ± 0.04 mg/mL). The methanol extract (IC50 = 0.05 ± 0.01 mg/mL) and the ethyl acetate fraction (IC50 = 0.01 ± 0.00 mg/mL) demonstrated stronger inhibitory activity against α-glucosidase than acarbose (IC50 = 0.27 ± 0.13 mg/mL). Phytochemical testing showed that the methanol extract and the ethyl acetate fraction contained secondary metabolites: alkaloids, flavonoids, steroids, and phenol hydroquinone. The compounds in methanol extract predicted to have inhibitory activity against α-amylase and α-glucosidase were Docosanol, Neophytadiene, Stigmasta-7,22-dien-3-ol,acetate,(3.beta.,5.alpha.,22E), Octadecanoic acid,2-oxo-,methyl ester, and phytol, while those in the ethyl acetate fraction were n-Nonadecane, Phytol, Butyl ester, 14-.Beta.-H-pregna, Octadecenoic acid, and Oleic acid. read more

In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed Halimeda tuna Extract from the Coast of Lhok Bubon, Aceh

Mohamad Gazali, Odi Jolanda, Amir Husni, Nurjanah, Fadzilah Adibah Abd Majid, Zuriat and Rina Syafitri

Abstract

Seaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed Halimeda tuna. The H. tuna sample was extracted with the maceration method using methanol and then partitioned using ethyl acetate and water to obtain ethyl acetate and water fractions. The methanol extract, ethyl acetate fraction, and water fraction of H. tuna were tested for their inhibitory activity against α-amilase and α-glucosidase. The methanol extract and the fractions with the highest inhibitory activity were phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the ethyl acetate fraction (IC50 = 0.88 ± 0.20 mg/mL) inhibited α-amylase relatively similar to acarbose (IC50 = 0.76 ± 0.04 mg/mL). The methanol extract (IC50 = 0.05 ± 0.01 mg/mL) and the ethyl acetate fraction (IC50 = 0.01 ± 0.00 mg/mL) demonstrated stronger inhibitory activity against α-glucosidase than acarbose (IC50 = 0.27 ± 0.13 mg/mL). Phytochemical testing showed that the methanol extract and the ethyl acetate fraction contained secondary metabolites: alkaloids, flavonoids, steroids, and phenol hydroquinone. The compounds in methanol extract predicted to have inhibitory activity against α-amylase and α-glucosidase were Docosanol, Neophytadiene, Stigmasta-7,22-dien-3-ol,acetate,(3.beta.,5.alpha.,22E), Octadecanoic acid,2-oxo-,methyl ester, and phytol, while those in the ethyl acetate fraction were n-Nonadecane, Phytol, Butyl ester, 14-.Beta.-H-pregna, Octadecenoic acid, and Oleic acid. read more

Optimization Concentration of Irgacure® 2959 as Photo-initiator on Chitosan-Kappa-Carrageenan Based Hydrogel for Tissue Sealant

Fiskina Zulfa Kamala, Wiratni Budhijanto, Eko Purnomo, Prihati Sih Nugraheni

Hydrogel is a three-dimensional network of hydrophilic polymers widely used in the biomedical field, one of which is a tissue sealant. The material that can be used in the manufacture of hydrogels is chitosan. The ability of chitosan in the formation of hydrogel is still limited by its swelling and adhesive properties, so it requires other materials such as crosslinkers, one of which is kappa-carrageenan which can stabilize and increase the viscosity of the hydrogel. The addition of Irgacure^® 2959 as a photo-initiator to the hydrogel can produce free radicals that can bind to the active group of the polymer. This study aims to determine the effect of adding Irgacure^® 2959 in the manufacture of hydrogel based on a chitosan-kappa-carrageenan solution formulated as a colon tissue adhesive. The treatments given were the addition of Irgacure^® 2959 as much as 0%, 1%, 2%, 3%, and 4%. The characteristics of the hydrogel tested qualitatively (simulation adhesion test), degree of swelling, resistance to water, degree of crystallinity, and ability to inhibit bacterial growth. The addition of Irgacure^® 2959 did not show significantly different results from the control that indicating it cannot increase the crosslinking between chitosan and kappa-carrageenan. This is predicted due to the presence of steric hindrance from kappa-carrageenan, causing very limited crosslinking due to the molecular size difference between chitosan and kappa-carrageenan. Further, it is necessary to optimize the ratio of the chitosan and kappa-carrageenan to get the balance ratio that supports the occurrence of cross-linking. read more

Optimization of Irgacure® 2959 Concentration as Photo-Initiator on Chitosan-Alginate Based Hydrogel for Colon Tissue Sealant

Diah Puspitasari, Wiratni Budhijanto, Eko Purnomo, Prihati Sih Nugraheni

Tissue sealant is a material that is used as an adhesive to bond two tissue surfaces used during surgery. Chitosan and alginate are natural macromolecules used to manufacture hydrogels as tissue sealants because these two materials can strengthen bonds and the mechanical properties of tissue sealants. Irgacure^® 2959, as a photo-initiator, can strengthen the cross-linking between chitosan-alginate to produce a tissue sealant with good mechanical strength. Therefore, this study aims to determine the effect of adding Irgacure^® 2959 to chitosan-alginate hydrogel with different concentrations as a tissue sealant designed for the colon. The experimental design used a completely randomized design (CRD) with the addition of Irgacure^® 2959 0; 1; 2; 3; 4%. The characteristics of the hydrogel as a tissue covering observed in this study include the degree of swelling, water resistance, simulated adhesion, degree of crystallinity, and ability to inhibit bacteria. The results of this study showed that the addition of 4% Irgacure^® 2959 reduced the degree of swelling, increased the hydrogel’s resistance to water, and increased adhesion. The interaction of chitosan and alginate with the addition of Irgacure^® 2959 produces cross-links, as evidenced by a degree of crystallinity of 26.21%. Based on these results, the chitosan-alginate hydrogel with the addition of Irgacure^® 2959 showed good potential in tissue sealant formulations, even though the inhibition of bacteria in all treatments showed low results, namely < 5 mm. read more

In order to play an active role in disseminating research results in the field of fishery product processing, lecturers of the Fisheries Product Technology Study Program participated in the 2022 International Fisheries Symposium (IFS). IFS this year was held on 5-6 December 2022 at Nha Trang University Vietnam . On this occasion the THP Study Program lecturers consisting of Prof. Dr.Sc. Amir Husni, Dr. Siti Ari Budhiyanti, Dr. Latif Sahubawa, Dr. Nurfitri Ekantari, Dr. Prihati Sih Nugraheni and Mgs. Muhammad Prima Putra, Ph.D. participated in these activities as a presenter both oral and poster. During the inauguration night IFS 2022, Mgs. Muhammad Prima Putra, Ph.D. was awarded the Best Oral Presenter award in the fishery product processing class (East Asia Fisheries and Technologist Association (EAFTA)). See you at IFS 2023 which is planned to be held at the Asian Institute of Technology (AIT) Bangkok Thailand. read more

In 2022, FPT-SP actively publish in both national and international reputated journal as follows:

Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology read more

Pada sepanjang tahun 2022, dosen prodi THP aktif dalam menghasilkan publikasi baik di jurnal nasional maupun jurnal internasional bereputasi sebagai berikut:

The effect of different drying methods on antioxidant compounds and fucoxanthin content of brown seaweed from the intertidal zone of West Aceh, Indonesia read more

Dalam rangka berperan aktif dalam mendeseminasikan hasil-hasil penelitian dibidang pengolahan hasil perikanan, dosen-dosen Prodi Teknologi Hasil Perikanan mengikuti International Fisheries Symposium (IFS) tahun 2022. IFS pada tahun ini diselenggarakan pada tanggal 5-6 Desember 2022 bertempat di Nha Trang University Vietnam. Dalam kesempatan ini dosen-dosen Prodi THP yang terdiri dari Prof. Dr.Sc. Amir Husni, Dr. Siti Ari Budhiyanti, Dr. Latif Sahubawa, Dr. Nurfitri Ekantari, Dr. Prihati Sih Nugraheni dan Mgs. Muhammad Prima Putra, Ph.D. mengikuti kegiatan tersebut sebagai presenter baik oral maupun poster. Pada kegiatan IFS 2022 ini, Mgs. Muhammad Prima Putra, Ph.D. mendapatkan penghargaan Best Oral Presenter pada kelas pengolahan hasil perikanan (East Asia Fisheries and Technologist Association (EAFTA)). Sampai bertemu pada IFS 2023 yang rencananya akan dilaksanakan di Asian Institute of Technology (AIT) Bangkok Thailand. read more

Prodi Teknologi Hasil Perikanan pada penghujung tahun 2022 sangat berbahagia dengan bertambahnya 1 orang guru besar yaitu Prof. Dr.Sc. Amir Husni, S.Pi., M.P. Dengan bertambahnya 1 orang guru besar ini, Prodi THP memiliki 2 orang guru besar. Atas nama Prodi, mengucapkan selamat dan sukses kepada Prof. Dr.Sc. Amir Husni, S.Pi., M.P. semoga semakin meningkatkan peran dalam dunia pengolahan hasil perikanan.

Di penghujung tahun 2022, mahasiswa prodi THP kembali memberikan kabar gembira. Alhamdulillah 2 tim gabungan dari prodi-prodi di perikanan baru saja memenangkan kompetisi

Tanggal 10 Nov 2022
Lomba: Economic & Business Entrepreneur Club FESTIVAL 2022

Hasil: JUARA 1

Anggota tim:
1. Muhammad Athallah Rumi (21/481260/PN/17433) Akuakultur
2. Galuh Wulanuari (21/481535/PN/17455) Akuakultur
3. Diana Febriyanti (21/482892/PN/17556) Teknologi Hasil Perikanan

Dosen Pembimbing: Dr. Nurfitri Ekantari

Tanggal 16 Nov 2022
Lomba gagasan inovasi tingkat perguruan tinggi (Penyelenggara FPIK IPB) read more