Antioxidant, antidiabetic activities and consumer acceptance of Sargassum hystrix tea combined with cinnamon powder

Setiyawan, A. and Husni, A.

Abstract
Sargassum hystrix is a species of brown seaweed containing bioactive compounds and has the potential to be used as a functional drink. This study was aimed to determine the fucoidan content, antioxidant, antidiabetic activities, and consumer acceptance rate of combining cinnamon powder to manufacture S. hystrix seaweed tea. The process was carried out by washing, drying, cutting, blending, weighing, roasting, and packaging the seaweed. Furthermore, the seaweed was tested to determine the total phenolic content, antioxidant (scavenging of hydroxyl radical and superoxide anion), antidiabetic (inhibition of α-glucosidase and α-amylase) activities and sensory evaluation. The results showed that the addition of cinnamon powder affected the total phenol content, antioxidant and antidiabetic activities, and consumer acceptance of S. hystrix tea. Meanwhile, an increase in the amount (0 ~ 5%) of cinnamon powder reduced levels of antioxidants (46.90±0.92 to 39.53±0.11% for hydroxyl radical scavenging activity, and 82.00±1.19 to 73.56±1.23% for superoxide anion scavenging activity), with a rise in antidiabetic activity (22.27±1.74 to 83.98±2.37% for inhibition activity of α-glucosidase and 72.94±1.55 to 95.83±1.06% for inhibition activity of α-amylase). In addition, the use of 5% and 2% cinnamon powder has the ability to overcome the fishy smell and taste, respectively. read more

Antioxidant, antidiabetic activities and consumer acceptance of Sargassum hystrix tea combined with cinnamon powder

Setiyawan, A. and Husni, A.

Abstract
Sargassum hystrix is a species of brown seaweed containing bioactive compounds and has the potential to be used as a functional drink. This study was aimed to determine the fucoidan content, antioxidant, antidiabetic activities, and consumer acceptance rate of combining cinnamon powder to manufacture S. hystrix seaweed tea. The process was carried out by washing, drying, cutting, blending, weighing, roasting, and packaging the seaweed. Furthermore, the seaweed was tested to determine the total phenolic content, antioxidant (scavenging of hydroxyl radical and superoxide anion), antidiabetic (inhibition of α-glucosidase and α-amylase) activities and sensory evaluation. The results showed that the addition of cinnamon powder affected the total phenol content, antioxidant and antidiabetic activities, and consumer acceptance of S. hystrix tea. Meanwhile, an increase in the amount (0 ~ 5%) of cinnamon powder reduced levels of antioxidants (46.90±0.92 to 39.53±0.11% for hydroxyl radical scavenging activity, and 82.00±1.19 to 73.56±1.23% for superoxide anion scavenging activity), with a rise in antidiabetic activity (22.27±1.74 to 83.98±2.37% for inhibition activity of α-glucosidase and 72.94±1.55 to 95.83±1.06% for inhibition activity of α-amylase). In addition, the use of 5% and 2% cinnamon powder has the ability to overcome the fishy smell and taste, respectively. read more

The impact of immersion time in lime solution on antioxidant and antidiabetic properties and consumer evaluation of Sargassum hystrix seaweed tea

Damayanti Rahmatika Pratiwi, Amir Husni

Abstract.

Sargassum hystrix is a brown seaweed that contains bioactive substances, such as antioxidant and antidiabetic compounds, and is used as a functional ingredient in the food industry. They are mostly found in Indonesian waters, growing along rocky shorelines. Since the beverages extracted from seaweed are found to be less preferred by consumers due to the fishy smell, efforts are needed to curtail this problem. Based on this condition, the lime solution immersion method is observed as a suitable approach to reduce the pungent smell. Therefore, this study aims to determine the effect of the immersion time of the lime solution on antioxidant and antidiabetic properties, as well as on consumers’ preference for S. hystrix tea. In this research, the variation of seaweed immersion time at 0, 4, 8, 12, and 16 min, was used in a lime solution of pH 5 at 85°C. Multiple analyses were further carried out on the beverage, including moisture and total phenol content, hedonic teat, and antioxidant property, such as inhibitions of DPPH (1,1-diphenyl-2-picrylhydrazyl), FRAP (ferric reduction antioxidant power), and α-glucosidase. The results indicated that the immersion time had no effect (p > 0.05) on the water content, although it significantly affected total phenol levels, antioxidant, and antidiabetic activities, as well as the consumer preference for S. hystrix tea (p < 0.05). The best quality was obtained at 16 min, which indicated water and total phenol contents at 3.10 ± 0.33% and 76.81±0.23 mg GAE g-1, with DPPH, FRAP, and α-lucosidase inhibition activities at 53.86±1.27%, 121.82±0.91 µM g-1 , and 52.86±0.89%, respectively. It also showed consumer evaluations for appearance, color, flavor, taste, and overall quality, at 4.39±0.91, 4.33±0.78, 4.08±1.23, 4.25±1.08, and 4.26+0.13, respectively. Furthermore, immersion time in lime solution improved the quality and consumer evaluation of S. hystrix tea. read more

Sebagai salah satu bentuk kewajiban dalam pelaksanaan tridharma perguruan tinggi, dosen-dosen Program Studi Teknologi Hasil Perikanan melaksanakan kegiatan pengabdian kepada Masyarakat. Kegiatan pengabdian ini difokuskan di kelmpok pengolah hasil perikanan (Poklahsar) Mina Manggal yang terletak di Purwomartani, Kalasan, Sleman Yogyakarta. Poklahsar Mina Manggal merupakan poklahsar yang terbentuk dari kelompok wanita tani (KWT) putri manggal yang telah berdiri sebelumnya dan aktiv dalam melaksanakan kegiatan-kegiatan perikanan dan pertanian. read more

The impact of immersion time in lime solution on antioxidant and antidiabetic properties and consumer evaluation of Sargassum hystrix seaweed tea

Damayanti Rahmatika Pratiwi, Amir Husni

Abstract. Sargassum hystrix is a brown seaweed that contains bioactive substances, such as antioxidant
and antidiabetic compounds, and is used as a functional ingredient in the food industry. They are mostly
found in Indonesian waters, growing along rocky shorelines. Since the beverages extracted from seaweed
are found to be less preferred by consumers due to the fishy smell, efforts are needed to curtail this problem. Based on this condition, the lime solution immersion method is observed as a suitable approach to reduce the pungent smell. Therefore, this study aims to determine the effect of the immersion time of the lime solution on antioxidant and antidiabetic properties, as well as on consumers’ preference for S. hystrix tea. In this research, the variation of seaweed immersion time at 0, 4, 8, 12, and 16 min, was used in a lime solution of pH 5 at 85°C. Multiple analyses were further carried out on the beverage, including moisture and total phenol content, hedonic teat, and antioxidant property, such as inhibitions of DPPH (1,1-diphenyl-2-picrylhydrazyl), FRAP (ferric reduction antioxidant power), and α-glucosidase. The results indicated that the immersion time had no effect (p > 0.05) on the water content, although it significantly affected total phenol levels, antioxidant, and antidiabetic activities, as well as the consumer preference for S. hystrix tea (p < 0.05). The best quality was obtained at 16 min, which indicated water and total phenol contents at 3.10 ± 0.33% and 76.81±0.23 mg GAE g-1, with DPPH, FRAP, and α-glucosidase inhibitionactivities at 53.86±1.27%, 121.82±0.91 µM g-1 , and 52.86±0.89%, respectively. It also showed consumer evaluations for appearance, color, flavor, taste, and overall quality, at 4.39±0.91, 4.33±0.78, 4.08±1.23, 4.25±1.08, and 4.26+0.13, respectively. Furthermore, immersion time in lime solution improved the quality and consumer evaluation of S. hystrix tea.
Key Words: Bioactive, brown seaweed, consumer preference, fishy smell, functional food. read more

Mohamad Aji Ikhrami1, Jaka Widada2, Indun Dewi Puspita1 and Masagus Muhammad
Prima Putra1*

1Fish Product Technology, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah
Mada, Jl. Flora gedung A4, Bulaksumur Yogyakarta 55281
2Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jl.
Flora gedung A4, Bulaksumur Yogyakarta 55281
*Corresponding author, e-mail: primaputra@ugm.ac.id

ABSTRACT
Streptomyces is a Gram-positive bacteria that produces the largest secondary metabolite
compounds. The results of whole-genome sequence analysis showed that Streptomyces can carry
more than 30 Biosynthetic Gene Clusters (BGC) encoding secondary metabolites that have the
potential to be explored in the exploration for new bioactive compounds. However, not all BGC
can be expressed in the laboratory scale and requires a specific activation method. This study aims
to explore the potential of chitosan as an elicitor compound to activate and or increase the
antibacterial activity of Streptomyces sp. GMR-22 was tested against histamine-producing bacteria
(HPB) Morganella morganii TK7 and Citrobacter freundii CK1. Chitosan was added to the
fermentation medium with the final concentration of 250, 500, and 750 μg/ml while without the
addition of chitosan used as control. Fermentation was carried out for 10 days at room temperature,
with constant agitation 200 rpm. The supernatant was separated by centrifugation at 3500 rpm for
15 minutes, then fractionation with ethyl acetate, concentrated by vacuum rotary evaporator, and
freeze-dried. The test for antibacterial activity was carried out by the microdilution method with
an extract concentration of 100 mg/ml. The test results of the microdilution method showed that
the addition of chitosan successfully increases the antibacterial activity with the highest activity
shown by the water fraction of 250 μg/ml addition of chitosan which is effective in inhibiting the
growth of Morganella morganii TK7 and Citrobacter freundii CK1 by 97,29% and 97,92%
respectively. read more

Mohamad Aji Ikhrami1, Jaka Widada2, Indun Dewi Puspita1 and Masagus Muhammad
Prima Putra1*

1Fish Product Technology, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah
Mada, Jl. Flora gedung A4, Bulaksumur Yogyakarta 55281
2Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Jl.
Flora gedung A4, Bulaksumur Yogyakarta 55281
*Corresponding author, e-mail: primaputra@ugm.ac.id

ABSTRACT
Streptomyces is a Gram-positive bacteria that produces the largest secondary metabolite
compounds. The results of whole-genome sequence analysis showed that Streptomyces can carry
more than 30 Biosynthetic Gene Clusters (BGC) encoding secondary metabolites that have the
potential to be explored in the exploration for new bioactive compounds. However, not all BGC
can be expressed in the laboratory scale and requires a specific activation method. This study aims
to explore the potential of chitosan as an elicitor compound to activate and or increase the
antibacterial activity of Streptomyces sp. GMR-22 was tested against histamine-producing bacteria
(HPB) Morganella morganii TK7 and Citrobacter freundii CK1. Chitosan was added to the
fermentation medium with the final concentration of 250, 500, and 750 μg/ml while without the
addition of chitosan used as control. Fermentation was carried out for 10 days at room temperature,
with constant agitation 200 rpm. The supernatant was separated by centrifugation at 3500 rpm for
15 minutes, then fractionation with ethyl acetate, concentrated by vacuum rotary evaporator, and
freeze-dried. The test for antibacterial activity was carried out by the microdilution method with
an extract concentration of 100 mg/ml. The test results of the microdilution method showed that
the addition of chitosan successfully increases the antibacterial activity with the highest activity
shown by the water fraction of 250 μg/ml addition of chitosan which is effective in inhibiting the
growth of Morganella morganii TK7 and Citrobacter freundii CK1 by 97,29% and 97,92%
respectively. read more

Theresia Adven D. K., mahasiswi Program Studi Teknologi Hasil Perikanan angkatan 2019 tahun ini berhasil menorehkan prestasi tingkat nasional. Yang pertama, There meraih Juara 1 Lomba Menulis Opini Tingkat Nasional dengan penyelenggara BPPM Primordia Fakultas Pertanian UGM dan yang kedua Juara 1 Lomba Videografi Tingkat Nasional dengan penyelenggara Panitia Lustrum XV Fakultas Pertanian UGM.

Pris Larasati1, Amir Husni2*
1Departemen Perikanan Fakultas Pertanian Universitas Gadjah Mada
2Pusat Kajian Ketahanan dan Keberlanjutan Hasil Laut Departemen Perikanan Fakultas Pertanian
Universitas Gadjah Mada

Abstrak

Rumput laut Sargassum crassifolium mempunyai bioaktivitas yang tinggi sehingga berpotensi sebagai sumber pangan fungsional, salah satunya dibuat menjadi teh rumput laut. Salah satu kelemahan teh rumput laut yaitu kurang disukai konsumen karena adanya bau amis. Penelitian ini bertujuan untuk mengetahui pengaruh lama perendaman dalam air 85oC terhadap karakteristik dan tingkat penerimaan konsumen teh rumput laut S. crassifolium. Rancangan acak lengkap digunakan dalam penelitian ini, dengan perlakuan berupa variasi lama waktu perendaman. Teh rumput laut dibuat dengan cara merendam dalam air ±85oC selama 0, 4, 8, 12, dan 16 menit kemudian dipotong-potong lalu disangrai. Teh rumput laut S. crassifolium dianalisis meliputi: kadar air, total fenol, aktivitas antioksidan dengan metode 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan Ferric Reducing Antioxidant Power (FRAP), aktivitas penghambatan α-glukosidase dan uji hedonik. Hasil penelitian menunjukkan bahwa lama perendaman berpengaruh nyata (p<0,05) terhadap kadar air, total fenol, aktivitas antioksidan, penghambatan α-glukosidase, dan tingkat penerimaan konsumen. Perlakuan terbaik yaitu lama perendaman 16 menit dengan kadar air 3,35±0,41%, total fenol 68,63±0,67 mg GAE/g, aktivitas penghambatan DPPH 55,99±1,01%, nilai FRAP 116,97±1,89 μM/g, penghambatan α-glukosidase 55,67±0,36%, dan nilai hedonik parameter aroma, rasa, warna, kenampakan, dan keseluruhan secara berturut-turut yaitu 4,29±1,18; 4,59±0,85; 4,18±0,98; 4,43±0,91; 4,37±0,98. read more